Genome-Wide Analysis of MAMSTR Transcription Factor-Binding Sites via ChIP-Seq in Porcine Skeletal Muscle Fibroblasts
نویسندگان
چکیده
Myocyte enhancer factor-2-activating motif and SAP domain-containing transcriptional regulator (MAMSTR) regulates its downstream through binding in promoter regions. However, molecular mechanism, particularly the DNA-binding sites, coregulatory genes are quite unexplored. Therefore, to identify genome-wide sites of MAMSTR transcription factors their genes, chromatin immunoprecipitation sequencing was carried out. The results showed that associated with 1506 peaks, which were annotated as 962 different genes. Most these involved regulation, metabolic pathways, cell development differentiation, such AMPK signaling pathway, TGF-beta coactivator activity, binding, adipocytokine fat digestion absorption, skeletal muscle fiber development, differentiation. Lastly, expression levels activities PID1, VTI1B, PRKAG1, ACSS2, SLC28A3 screened verified via functional markers analysis. Overall, this study has increased our understanding regulatory mechanism during fibroblast provided a reference for analyzing mechanisms.
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ژورنال
عنوان ژورنال: Animals
سال: 2023
ISSN: ['2076-2615']
DOI: https://doi.org/10.3390/ani13111731